human cc cell lines  (ATCC)

Journal: Open Life Sciences

Article Title: Effects and potential mechanisms of luteolin on cisplatin mediated inhibition of cervical adenocarcinoma

doi: 10.1515/biol-2025-1274

Figure Lengend Snippet: Luteolin inhibits the proliferation and colonization capacity of HeLa cells. HeLa cells were treated with different concentrations of LU (0, 0.1, 0.5, 5, 10, 20, 40 µM 0 μM) and CDDP (0, 0.5, 2.5 μg/mL) for 24 h and 48 h. A and B. HeLa cell proliferation induced by different concentrations of LU and CDDP for 24 h and 48 h was detected by CCK8 assay. C and D. Siha cell proliferation induced by different concentrations of LU and CDDP for 24 h and 48 h was detected by CCK8 assay. E and F. The number and morphology of hela and siha cells were observed with microscope. G and H. HeLa cell proliferation was detected by colony formation assay and statistical analysis. * P < 0.05, ** P < 0.01 compared with the control group.

Article Snippet: The human cervical cancer HeLa and Siha cell lines were obtained from Procell Life Science & Technology Co., Ltd.

Techniques: CCK-8 Assay, Microscopy, Colony Assay, Control

Journal: Oncology Letters

Article Title: Lactylation-based machine algorithm combined with multi-omics analysis to predict prognosis in cervical cancer

doi: 10.3892/ol.2026.15486

Figure Lengend Snippet: HMGN1 inhibition affects the proliferation of cervical squamous cells. (A) mRNA and (B) protein expression levels of HMGN1 in SiHa and HeLa cells following siRNA transfection, compared with the NC. (C) Cell Counting Kit-8 assay assessing the proliferation of SiHa and HeLa cells at 24, 48 and 72 h. (D) Cell cycle distribution of SiHa and HeLa cells after HMGN1 inhibition was analyzed by flow cytometry, including flow cytometry plots and quantitative histograms. *P<0.05, **P<0.01 and ****P<0.001. HMGN1, high-mobility group nucleosome-binding protein 1; NC, non-targeting control; si, small interfering RNA.

Article Snippet: Human cervical squamous cell carcinoma cell lines SiHa (cat. no. HTB-35) and HeLa (cat. no. CRM-CCL-2) were obtained from the American Type Culture Collection and the human keratinocyte cell line HaCaT was obtained from The Cell Bank of Type Culture Collection of the Chinese Academy of Sciences (SCSP-5091; Beijing, China).

Techniques: Inhibition, Expressing, Transfection, Cell Counting, Flow Cytometry, Binding Assay, Control, Small Interfering RNA

Journal: Biology Direct

Article Title: FAM83D facilitates EMT and metastasis of cervical cancer via interaction with GSK3β and inactivation of GSK3β/stabilization of Snail signaling

doi: 10.1186/s13062-026-00761-z

Figure Lengend Snippet: FAM83D was associated with metastasis of CC. ( A ) Western blotting was employed to examine the expression of FAM83D in HeLa and SiHa cells with stable overexpression or knockdown of FAM83D. ( B-C ) Colony formation assay was conducted to evaluate the clonogenic ability of HeLa and SiHa cells following the overexpression or knockdown of FAM83D. ( D ) CCK-8 assay was used to detect cell viability in FAM83D-silenced HeLa and SiHa cells treated with different concentrations of 5-fluorouracil, cisplatin, oxaliplatin, and paclitaxel for 48 h. The inhibition rate was subsequently calculated. ( E-I ) Subcutaneous injection of FAM83D-silenced and control HeLa cells into BALB/c Nude mice ( n = 6) was used to determine the tumorigenicity. The tumor growth curves, tumor volumes, and tumor weights were measured. ( J ) And then, the nude mice that developed liver metastases were counted. ( K ) Representative images of liver metastases developed from FAM83D-silenced and control HeLa cells were shown, while ( L ) H&E staining of liver metastases in nude mice was presented. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human CC cell lines SiHa and HeLa were purchased from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Expressing, Over Expression, Knockdown, Colony Assay, CCK-8 Assay, Inhibition, Injection, Control, Staining

Journal: Biology Direct

Article Title: FAM83D facilitates EMT and metastasis of cervical cancer via interaction with GSK3β and inactivation of GSK3β/stabilization of Snail signaling

doi: 10.1186/s13062-026-00761-z

Figure Lengend Snippet: FAM83D promoted the metastasis of CC cells in vitro and vivo. ( A-D ) Wound healing assay was conducted to determine the migratory ability, and ( E-H ) transwell assay was utilized to determine the migratory and invasive ability, respectively, in HeLa and SiHa cells exhibiting stable overexpression or silencing of FAM83D. ( I ) Lung metastasis in nude mice ( n = 6) by tail vein injection of HeLa cells with or without silencing of FAM83D. ( J-K ) The statistical data of lung metastasis occurring in the mouse model. ( L ) Representative images and H&E staining of lung metastasis in nude mice. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human CC cell lines SiHa and HeLa were purchased from the American Type Culture Collection (ATCC).

Techniques: In Vitro, Wound Healing Assay, Transwell Assay, Over Expression, Injection, Staining

Journal: Biology Direct

Article Title: FAM83D facilitates EMT and metastasis of cervical cancer via interaction with GSK3β and inactivation of GSK3β/stabilization of Snail signaling

doi: 10.1186/s13062-026-00761-z

Figure Lengend Snippet: FAM83D promoted GSK3β/Snail signaling and EMT. ( A-D ) Western blotting for E-cadherin, N-cadherin, Vimentin, and FAM83D in HeLa and SiHa cells following stable overexpression or silencing of FAM83D. ( E-H ) Western blotting for Snail, β-catenin, GSK3β, and phosphorylated GSK3β (Ser9), and FAM83D protein changes in CC cells with stable overexpression or silencing of FAM83D. ( I-J ) Cycloheximide (CHX) chase assays for Snail stability in CC cells with stable overexpression of FAM83D. ( K-L ) Ubiquitination assay for Snail protein ubiquitination level in CC cells with stable overexpression or silencing of FAM83D in the presence or absence of LiCl. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human CC cell lines SiHa and HeLa were purchased from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Over Expression, Ubiquitin Proteomics

Journal: Biology Direct

Article Title: FAM83D facilitates EMT and metastasis of cervical cancer via interaction with GSK3β and inactivation of GSK3β/stabilization of Snail signaling

doi: 10.1186/s13062-026-00761-z

Figure Lengend Snippet: FAM83D interacted with GSK3β. ( A ) The possible interaction between FAM83D and GSK3β was screened from the IntACT database. ( B-D ) Co-IP assay for interaction between FAM83D and GSK3β in HeLa and SiHa cells with or without silencing of FAM83D. ( E-F ) Co-IP assay for interaction between FAM83D and phosphorylated GSK3β (Ser9) in HeLa and SiHa cells. ( G ) In vitro cell-free proteins binding assay for validation of interaction specificity between FAM83D and GSK3β. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human CC cell lines SiHa and HeLa were purchased from the American Type Culture Collection (ATCC).

Techniques: Co-Immunoprecipitation Assay, In Vitro, Binding Assay, Biomarker Discovery

Journal: Biology Direct

Article Title: FAM83D facilitates EMT and metastasis of cervical cancer via interaction with GSK3β and inactivation of GSK3β/stabilization of Snail signaling

doi: 10.1186/s13062-026-00761-z

Figure Lengend Snippet: The role of GSK3β/Snail axis in FAM83D-regulated EMT and metastasis of CC. ( A-D ) Western blotting for expressions of E-cadherin, N-cadherin, Vimentin, Snail, GSK3β, p-GSK3β (Ser9), and FAM83D in CC cells following stable FAM83D knockdown, as well as treated with LiCl (10 mM and 15 mM in HeLa and SiHa cells, respectively) for 24 h. ( E-H ) Following the treatment of HeLa cells with LiCl (10 mM) for 24 h and SiHa cells with LiCl (15 mM) for 24 h, a transwell assay was conducted to assess the migratory and invasive capabilities of HeLa and SiHa cells with stable FAM83D knockdown. The counts of migratory and invasive cells were recorded and subjected to statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: Human CC cell lines SiHa and HeLa were purchased from the American Type Culture Collection (ATCC).

Techniques: Western Blot, Knockdown, Transwell Assay